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E-Cadherin cell staining was defined as high (>33%) or low (<33%), and values were assigned by a pathologist blinded to the tumor characteristics.
For ER and PR, the Allred scores (0−8) assigned by a pathologist were translated into negative for 0 score, weak for 2 and 3 scores, intermediate for 4, 5, and 6 scores, and strong for 7 and 8 scores.
The histological subtypes are defined through the three-digit pathohistological diagnosis code assigned by a pathologist.
Third, we also used as a 'regulator' a clinical parameter, the Gleason grade, a discrete score assigned by a pathologist based on the microscopic appearance of prostate tissue biopsies.
To further determine the correlation between tissue pathology and the integrated intensity of the fluorescence signal, a detailed analysis was performed on several tissue specimens that were assigned by a pathologist to one of the next classes: normal colon, adenomas, and adenocarcinomas.
Spearman's correlation coefficient was used to assess correlations between the Citizen Scientist's performance score and the number of cores scored by each Citizen Scientist and between the Citizen Scientist's ER scores for each TMA core and the Allred score assigned by a pathologist.
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Scores were assigned by a 5% increment from 0to100%0% and by two independent pathologists who were blinded to the clinical follow-up data.
Disease stage was assigned by a gynecologic-oncologist, and tumour grade and histopathological subtypes were assigned by a gynecologic-oncologist pathologist according to the criteria established by the International Federation of Gynecology and Obstetrics.
The primary causes of death were assigned by the pathologist based on integration of the clinical information and the gross and microscopic findings from the autopsy.
We then compared the ER staining as measured by the Allred score assigned by the pathologist with the Citizen Scientist pseudo-Allred score for the subset of 2121 cores with cancer cells identified by both the pathologist and the Citizen Scientists.
If a malignancy was found, the lesion was removed surgically by lumpectomy or mastectomy, and a final pathological stage was assigned by the pathologists in the usual manner for that hospital in accordance with the American Joint Committee on Cancer (AJCC) TNM system guidelines.
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