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We assigned breeding pairs randomly to two parental care treatments: Full Care, in which parents remained in the breeding boxes with the larvae until their dispersal and Prehatching Care, in which parents were removed from the breeding boxes 53 h after pairing.
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The n A -dimensional vector a A contains breeding values for purebred performance for breed animals (mating within breed ), and matrix Z A is an incidence matrix assigning breeding values to records.
Parentage was assigned by breeding monitoring [28] and mini/microsatellite analysis [14], [15], [29, Robertson unpublished data].
Forty separate spawning groups were established (20 controls and 20 exposed; see Fig. 2 Experiment I) and each group, comprising one female and two males (either all inbred or all outbred), were randomly assigned to breeding aquaria.
Before we assigned the breeding pairs, we genotyped mice to exclude individuals carrying t alleles, since these alleles cause meiotic drive and may influence females' mating preferences (Lenington, 1991).
Using all 18 locations as potential sites of origin, we found that only 30% of individuals were correctly assigned to their breeding site of origin using δD alone (figure 6a) and only 32% when using 87Sr/86Sr alone (figure 6b).
As the process of assigning a mosquito breeding site abundance index to each cell does not rely on fitting-to-data, validation of this methodology was conducted using available trapping data from Cairns for the years 2006 2013, kindly provided by Scott Ritchie James Cookk University and Peter Cook Monash Universityty).
With the introduction of Pokémon breeding, Pokémon are assigned to one or two breeding groups.
Other nesting habitat categories were not mutually exclusive and a score of 1 was assigned to additional breeding habitat variables to describe the nesting preferences of the species.
One of the resulting groups contained all the materials from breeding companies assigned to P3, and was separated from all remaining accessions from the study.
Indeed, elemental analyses of feathers moulted in wintering areas were more successful in assigning birds to their breeding origin than to their wintering areas (64.0% vs. 33.4% of correct classification; Table 1).
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