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A large portion of a real genomic sequence (even whole chromosomes of human or other species) can be assigned as a reference genome for a model population.
The level of resistin mRNA in healthy controls was assigned as a reference value of 1. Relative expression of resistin to the internal control was calculated using a 2-△△Ct method [ 15].
The Biacore 4000 fluidics system allows the assignment of five detection spots to each of four flow cells, enabling the screening of four different compounds per cycle, where one of the targets per flow cell has to be assigned as a reference or preferably left blank.
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The gene activity in the shame group was assigned as 1 as a reference.
Ferulic acid CRM has been approved and assigned as a primary reference material by the General Administration of Quality Supervision, Inspection, and Quarantine of the People׳s Republic of China.
For analyses of categorical obese-years, participants who were never obese during study follow-up (zero obese-years) were assigned as a comparator (reference category) and the level of obese-years was grouped into short, medium long and extensive periods representing 1 24.9, 25 49.9, 50 74.9 and ≥75 obese-years.
Patients with haematological cancer were assigned as the reference tumour group as the largest group (breast) did not have a representative age and gender distribution.
It is a sample by sample process where the first cycle data is assigned as the reference cycle data, and computation starts from the second cycle.
SNP imputed dosage was modelled as a linear covariate (on a continuous scale from 0 to 2) where one allele was assigned as the reference allele and the other allele the risk allele.
The most common allele in the controls was assigned as the reference category.
Since this is the French official method for monitoring muscle samples, it was assigned as the reference method.
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