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To overcome the difficulties in identifying tooth structures due to superimposition of 3D structures onto a 2D plane, distortion projection errors, and blurred images, blinded and not blinded assessments of root length and resorptions were performed.
Assessments of root caries and restorations indicated whether one or more lesions (restorations) were present in the mouth and were recorded as a "whole-mouth" dichotomous score for each condition.
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Detailed topological assessment of root architecture, branching, etc., was not carried out.
Assessment of root shape and length is an essential component of the initial diagnosis stage in orthodontics.
(B) Reconstruction of the sagittal slices produced a 3-D representation of the entire root structure to allow assessment of root volume (RV).
Assessment of root canal fillings is mostly by means of panoramic or periapical radiographs.
Assessment of root traits in crop breeding material can be slow and expensive, involving a combination of field-, glasshouse- and laboratory-based screens (Clark et al., 2011).
Quantitative assessment of root penetration by F. oxysporum germ tubes during the initial 12 24 h after inoculation was performed using scanning electron microscopy (Perez-Nadales and Di Pietro, 2011).
Tailored single sessions would mean less in-depth assessment (of root causes of complaints) and more emphasis on a problem-focused approach where the objective is to provide direct support to the client [ 34].
Since the assessment of root traits in plants can be slow and expensive, we employed an agar based high-throughput root phenotyping screen to characterize the root traits of a large B. napus mapping population and identify genetic loci controlling these traits under low Pi availability (Fig. 2 and Table 2).
For assessment of root growth, seed of the wild types and mutant lines were surface-sterilized (see below) and grown on basal full strength 1× Murashige and Skoog (MS) medium without vitamins (Cat.# MSP01, Caisson Laboratories, Inc., North Logan, UT), containing 1% Phytoblend (Cat.# PTP01, Caisson Laboratories) in omni trays (Fisher Scientific, Pittsburgh, PA) as described.
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