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Base calling and quality assessment were determined using the Phred software [ 24].
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Coagulation assessment was determined using functional methods and chromatometric assays.
Diagnosis at initial assessment was determined using what is generally considered the gold standard diagnostic interview for EDs, the Eating Disorder Examination (EDE) [ 26].
Pharmacogenetic testing was performed at the initial screening visit and baseline patient assessments were determined using the 17-item Hamilton Rating Scale for Depression (HAM-D17) and the Hamilton Rating Scale for Anxiety (HAM-A).
Objective tumor assessments were determined using RECIST, version 1.1.
Response assessments were determined using the SWOG criteria (Green and Weiss, 1992).
All final assessment measures were determined using last observation carried forward (LOCF).
Moreover, both Acute Physiology and Chronic Health Evaluation (APACHE) II score and Sequential Organ Failure Assessment (SOFA) score were determined using the worst values within the initial 24 hours of ED admission for disease severity assessment.
Accuracy assessments of maps were determined using error matrix and Kappa statistic (Congalton and Green 1999).
Function assessments on disability level were determined using the Lysholm knee scoring scale (0 100) [ 8] and the WOMAC Hip and Knee Osteoarthritis Index (0 100) [ 9, 10].
Assessment of VEGF-Axxxa isoform concentrations were determined using the ELISA protocol above, whereas VEGF-Axxxb isoforms were assessed by ELISA as described in [ 6]. 2 × 10 LS174t colon cancer cells were prepared after trypsinisation in 0.2 ml PBS and injected subcutaneously in the dorsum of 6 nude Balb/C mice for each group.
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