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The measurement properties of the CEAI, including a factor analysis and reliability assessment, were determined.
Lesions with pathologically increased FDG uptake obtained in the visual response assessment were determined by SUVmax using a "region of interest" (ROI) feature of a dedicated software tool (rover® v2.0.31, ABX GmbH, Radeberg, Germany).
Criteria for quality assessment were determined a priori (Table 1).
Base calling and quality assessment were determined using the Phred software [ 24].
Post-administration time-points for behavioural assessment were determined in preliminary experiments.
Inter-rater agreement and time needed to complete the assessment were determined.
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The immunogenicity assessment was determined by serum IgG responses to M2e and HAI was assessed before vaccination on Day 0 and on Days 14 and 28 after vaccination.
Macrophage death assessment was determined by incubating macrophages and yeast cells, as previously described, and cells stained with 1 µg/ml propidium iodide (PI) after 1, 2, 3, and 4 h of incubation.
Coagulation assessment was determined using functional methods and chromatometric assays.
Clinical assessment was determined in accordance with the definitions of the Centers for Disease Control and Prevention for nosocomial infections [ 20].
The precision of breakpoints assessment is determined by the resolution of an array.
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