Sentence examples for assessment of sequences from inspiring English sources
The occurrence of PCR recombination and mutations in sequences from gene families warrants scrutinised assessment of sequences.
For the initial assessment of sequences diversity we included all sequence variants which occurred in at least two independent PCR reactions, in each represented by at least three reads.
The ITS2 region was subsequently assessed using three different approaches: 1) rudimentary assessment of sequence variation was obtained by RFLP analysis; 2) intragenomic copy variants present were observed by heteroduplex analysis; and 3) DNA sequence variation was detailed through cloning and sequencing of the ITS2.
The advantage of the ASRT task is that it enables separate parallel assessment of sequence-specific and general skill learning.
The development of fluorescent DNA binding dyes with improved saturation properties has allowed a more precise assessment of sequence variation based on the analysis of DNA melting curves.
Because physical map data provides invaluable independent assessment of sequence assemblies, both within BACs and for longer-range ordering of contigs, we generated an independent Brachypodium physical map and integrated it with genome sequence assemblies.
First, we included putatively homogeneous PCR products identical to the infective strain inferred from previous sequencing efforts [4], [12], to allow qualitative and quantitative assessment of sequencing error within context of the bases in the specific region studied.
Quantitative assessment of sequence conservation was performed using phastCons with background (non-constrained) rates calculated from each locus alignment separately using phyloFit (HKY85+Gap substitution model), both from the PHASTCONS package [44] (version v0.9.9.6b).
Bases and QC assessment of sequencing were generated by CASAVA 1.8.
The script htseq-qa is a simple tool for initial quality assessment of sequencing runs.
