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Segmentation of these membrane proteins on a cell-by-cell basis enables the quantitative assessment of localization for comparative analysis.
This poses a challenge for accurate assessment of localization inside of nuclei because the ER membrane is contiguous with the outer nuclear membrane (Hetzer, 2010).
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Building on previous work with Zn2+-containing neurons, this study highlighted the use of TSQ for selectively labeling Zn2+-rich regions of the central nervous system for both quantitative estimates of labile Zn2+ pools and qualitative assessments of localization.
To provide a more accurate assessment of food localization, we suggested two modified measures of self-sufficiency and import dependency, namely the localization ratio (LR) and the modified import dependency ratio (MIDR).
This part of the work is aimed at providing a simple technique for the assessment of vibration localization effects of this type.
In addition, to DOX-loaded liposomes, calcein-loaded liposomes were prepared for assessment of the localization in tumor-bearing mice.
This visual assessment of GSK3β localization within the subcellular structures of various brain cells may help in understanding the diverse role of GSK3β signaling in the brain.
Presumably a quantitative assessment of nuclear localization?
In summary, the combination of polarization and dispersion of mRNA distribution enables an effective assessment of mRNA localization.
To allow assessment of surface localization, antibody-tags were introduced on both sides of the transmembrane domain.
Besides the assessment of spatial localization, we also performed immunohistochemical analysis using antibodies against well-established layer-specific neuronal markers (Greig et al., 2013; Kwan et al., 2012).
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