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XAlgPEG microbeads were found to be highly cell compatible with insulinoma cell lines, as well as rat and human pancreatic islets, where the viability and functional assessment of cells within XAlgPEG are comparable with Ba Alg controls.
After testing the multiplex assay on smears, our first aim was to establish the limits of the PNA FISH method for a quantitative assessment of cells in a membrane.
Recently developed techniques using injectable fluorescent tagged proteins allow functional assessment of cells in live animals [ 17].
Although histopathology is the main approach to gather such data, phenotypic assessment of cells in culture can be performed using high-content screening (HCS) methods.
Early toxicity assessment of cells in suspension can be determined for candidate-drugs treatment using a simple, low cost method that can provide rapid results.
Measurement of the extent of apoptosis was performed by assessment of cells appearing in a sub-G1 peak on DNA profiles.
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The MTS assay was used for the assessment of cell proliferation, and cell growth was assessed by counting live cells after staining with trypan blue.
Quantitative and morphological assessment of cell adhesion and proliferation for all cell types was assessed, after first elucidating an experimental composition range using MG63 cells.
Assessment of cell proliferation and cell apoptosis was estimated by histological, immunohistochemical, and Western blot analyses.
The integration of physical and cell culture characterization allows the comprehensive assessment of cell culture performance.
An assessment of cell proliferation was conducted using the WST-1 assay.
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