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The present study demonstrates long-term survival of CC cultures in vitro and describes a technique useful for rapid assessment of cell functionality and release properties of cultured monoaminergic cell types that later can be transplanted for neurotransmitter replacement following injury or disease.
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Both assays revealed significant and comparable loss of cell functionality that was confirmed by DNA assay.
It is worth mentioning that GO terms contain different types of conceptualizations of cell functionality.
The mitochondrion is a critic sensor of cell functionality and survival [ 23, 24].
Cell viability was determined by microscopic assessment of cell numbers and cell morphology (Cell-IQ assay).
Cell viability following oxidative insult was monitored by microscopic assessment of cell morphology and cell numbers using a Cell-IQ 2 automated live cell imaging platform (Chip-Man Technologies Ltd).
In summary, here we provide a detailed analysis of T cell functionality in stroke patients.
Our results also showed substantial inhibition of donor cell functionality by irradiation.
To provide a more complete assessment of the functionality of CD8+ T-cells expressing cognate T-cell receptors (TCR), measurement of CD107a and b expression can be combined with MHC-class I tetramer labeling and ICS [ 35].
This study was carried out to assess the impact of alloplasmic lines on cell functionality.
Finally, since the ability to prime and stimulate T cell proliferation is an important assessment of the functionality of DCs [25], we performed a mixed leucocyte reaction (MLR) to determine the proliferation of allogeneic lymphocytes induced by mature DCs.
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