Quantitative and morphological assessment of cell adhesion and proliferation for all cell types was assessed, after first elucidating an experimental composition range using MG63 cells.
Assessment of cell proliferation and cell apoptosis was estimated by histological, immunohistochemical, and Western blot analyses.
The integration of physical and cell culture characterization allows the comprehensive assessment of cell culture performance.
An assessment of cell proliferation was conducted using the WST-1 assay.
Therefore, apart from NP characterization, assessment of cell culture media must be made in order to understand its subsequent interaction with NPs.
This allows high signal-to-noise ratios using this nanobody-based imaging method for the long-term assessment of cell therapy.
A quantitative assessment of cell migration into the hydrogel network demonstrates a strong correlation between rate of cellular invasion and the network structure of the matrix.
In our experience the sensitivity of FACS allows for the detection of 5 fetal in 107 maternal cells and assessment of cell surface phenotype.
Finally we chose to evaluate three indicators of cell function: expression of a key transcription factor, expression of selected transcripts, and assessment of cell morphology.
Assessment of cell viability and contractility was performed using a Zeiss Axiovert 40 CFL inverted microscope.
For the assessment of cell death, three methodologies were used: Trypan Blue exclusion assay.
