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Although the ortholog hit ratio does not consider the effects of alternative splicing (unless alternative splice forms also exist in the reference dataset), it appears to be an excellent method for the comparative assessment of assemblies.
Beyond using highly conserved plant and eukaryotic genes for quality assessment of assemblies, we successfully focused on FLcDNA recovery and resolution of closely related sequences characteristic of large gene families of conifer defence and secondary metabolism.
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As more purpose built de-novo transcript assemblers become available there is a need for a systematic assessment of assembly tools and sequencing protocols for Illumina metatranscriptome data.
Assessment of assembly accuracy with homopolymer and read depth coverage generated with short reads.
Assessment of assembly quality and population homogeneity was conducted with MetaQUAST and CheckM, and final genome bins were curated as described above using RefineM35 to identify and filter outlier contigs from bins and to also employ k-means clustering to separate bins with multiple populations on the basis of various genomic properties (e.g., coverage profile, tetranucleotide frequencies).
Additional file 1: Assessment of assembly quality.
Secondly, our assessment of assembly quality found no evidence for selective amplification of unannotated sequences.
These datasets are not independent, but each offers a different assessment of assembly quality.
The map also aids in assembly of reference genomes, gap-filling, and independent assessment of assembly accuracy.
The preliminary 4× scaffold assembly was a "test run" produced by the sequencing consortium for an initial assessment of assembly characteristics of the genome.
Assessment of assembly completeness by CEGMA software showed 237 out of 248 ultra-conserved core proteins were 'complete' in the transcriptome, yielding a completeness of 95.6 %.
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