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A detailed characterization of the mouse model under the above conditions, including a histological assessment, gene expression analysis, and oil red o staining, is shown elsewhere (Meletta et al., manuscript submitted).
For quantitative assessment, gene expression was evaluated by quantitative RT-PCR (qRT-PCR) using the LightCycler CYBR Green Master kit (Roche Diagnostics, Mannheim, Germany) for cDNA amplification of specific target genes.
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LE carried out the experimental designs, disease assessments, gene expression studies, cloning, transformations, data analysis, and drafted the manuscript.
Proper assessment of RNA integrity is essential for reliable assessment of gene expression levels, as RNA molecules are acutely vulnerable to degradation.
Gene expression profiles based on a genome-wide assessment of gene expression represent a highly sensitive means of determining differences between cells or tissues.
To assess expression in living humans, these studies have been extended to the assessment of gene expression via analysis of mRNA from circulating mononuclear cells in whole blood [ 75, 76].
Exon array assessment of gene expression.
This paper presents a performance assessment of gene expression programming for automatic circuit model identification in impedance spectroscopy.
Using plasma mRNA analysis as a novel research tool for the non-invasive in vivo assessment of gene expression in vascular beds, we hypothesised that atorvastatin lowers the plasma mRNA level from statin pleiotropy-target genes, and the reduction is independent of the reduction of low-density lipoprotein cholesterol (LDL-C).
MPSS provides a comprehensive assessment of gene expression by generating short sequence tags, each 20 bp long, produced from a defined position for each transcript.
This study provides a comprehensive assessment of gene expression for pancreatic tumors, the normal pancreas, and nonneoplastic pancreatic disease.
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