Sentence examples for assessment cells from inspiring English sources
Establish specialist country based assessment cells within the Department of Immigration and Citizenship with country information used for refugee assessments continually updated and regularly vetted.
For eicosanoid production assessment, cells were preincubated for 24 h in serum-free DMEM followed by specific treatments.
For autophagy assessment, cells were visualized at 40× magnification using a Olympus BX51fluorescence microscope (Olympus America, Inc, Melville, NY, USA) and the percentage of cells showing numerous GFP-LC3 puncta (>10 dots/cells) were scored as described previously [22].
For lymphocyte subset assessment, cells were incubated with anti-CD4-Alexa Fluor 700 (2 µg/mL; BD Pharmingen), anti-CD8-PerCp-Cy5.5 (2 µg/mL, eBioscience), CD3 PE-Cy5 (2 µg/mL; BD Pharmingen), anti-FoxP3-PE (2 µg/mL, eBioscience), and anti-IL10-PE as previously shown [3].
For viability assessment, cells were cultured in flat-bottom 96-well plates.
For protein expression assessment, cells were harvested and conditioned medium supplemented with protease inhibitors cocktail (Roche, Vilvoorde, Belgium) were stored at -80°C until use.
Before in vivo assessment, cell proliferation and migration was compared in A375 shRNA control cells vs A375 shRNA SRPK1-transduced cells.
For phosphorylated MT1-MMP assessment, cell lysates were immunoprecipitated with MT1-MMP antibodies (MAB3328) prior to Western blot procedure and immunodetection with pMT1-MMP polyclonal antibodies [ 29].
For intracellular H2O2 assessments, cells were transfected with the Hyper-3 construct or the Hyper-CS control pH probe using Effectene according to the manufacturers' instructions (Qiagen, Manchester, UK).
For intrinsic cytotoxicity assessments, cells were incubated with varying concentrations of APE1 inhibitors and the MTS assay was performed on day 5.
Detailed descriptions of the routine maintenance of all applied cell cultures is available in the Supplemental Material ["Cell culture and assessment of cell viability" (http://www.ehponline.org/members/2008/0800121/suppl.pdf)].
