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For the purpose of assessing whether the increased level of TXNIP RNA determined by prolonged conditions of hyperglycemia (20 mM, chronic conditions) were correlated with correspondent elevated levels of TXNIP protein, we analyzed the expression of the protein in MDA-MB-231 cells grown "chronically" and stably in 20 mM glucose tissue culture media by Western blot.
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To assess whether the increased AP of Alk1 +/− mice could be mediated by increased production of NADPH-dependent superoxide in the CNS, we tested the effect of i.c.v.
Although we cannot conclusively assess whether the increased mass loss suffered by 'choosy' females is a direct result of the increased time spent with high-quality males, we suggest that this is the most likely explanation.
These analyses were important to assess whether the increased cathepsin activity was indicative of a general stimulation in lysosomal enzymes.
To assess whether the increased adhesive capacity of p190B transgenic MECs also affected cellular invasion, we quantified the number of invasive structures that formed when the primary MECs were grown in 3 D culture.
To assess whether the increased inflammation and destruction of the joints in Bid-/ mice was due to deficiencies in apoptosis, we examined the joints for apoptotic cells using TUNEL.
In a post hoc analysis to assess whether the increased incidence of bladder cancer was greater with even longer duration of exposure, we further subdivided the category of >24 months of exposure.
Our strategy was to assess whether the increased mortality risk among patients with depression can be reduced to the risk of patients who did not meet criteria for depression.
To assess whether the increased apoptosis by p110β silencing is caused by downregulation of BCL 2 in chemoresistant SKpac cells, TUNEL assay was performed after transfection of pcDNA3 BCL2 plasmid in p110β siRNA-treated SKpac cells.
As CD163 is a prototypical marker for MΦIL-10, we performed three types of experiments to assess whether the increased CD163 expression reflected a preferential M2 polarization in SpA.
The aim of this study was to evaluate the cost-effectiveness of one-sample and two-sample FIT screening strategies with variable intervals, age ranges and cut-off levels in order to assess whether the increased performance of a second FIT sample outweighs the increased costs compared with one-sample FIT screening.
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