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We have encountered significant difficulties, such as probe cross-reactivity and non-specific product detection, using conventional methods, in assessing the specificity of Tβ4 knockdown as against a non-specific effect on its closest relative Thymosin beta 10 (Tβ10).
For assessing the specificity of protein kinases for inhibitors, Ambit applied ATP site-dependent competition binding assays that directly and quantitatively measure the binding of inhibitors to the ATP binding site of kinases.
Besides assessing the specificity of each outcome, the presence or absence of a time frame was collected for every outcome.
Multiple primer pairs for each gene were tested and the most suitable primer candidates were chosen by assessing the specificity of the PCR product and efficiency.
Thus, we find these "negative" sites and their corollaries in the GABP dataset unreliable for assessing the specificity of the different programs using metrics such as a receiver operator curve (ROC), despite the fact that other groups have used this metric previously [12].
Thus, as another means of assessing the specificity of candidate APE1 inhibitors, and to begin to evaluate their potential biological value, we generated whole cell protein extracts from HEK 293T and HeLa cells and determined the effect of the most promising actives on total AP site cleavage activity [39].
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For the following specifications, we conducted 1,000 simulation experiments to assess the specificity and sensitivity of the statistic.
Having observed that the sera exhibit at least some neutralizing activity, we wanted to assess the specificity of the serum antibodies for oligomannose.
Four groups use a variety of methods in vitro and in cells to assess the specificity of genome editing with CRISPR-Cas systems (pp 822, 827, 833, 839).
To assess the specificity of this pathogen detection system, we employed a two-step experimental strategy.
This study assessed the specificity and potential of camels immunised with venom of medically most important snake of Western India, the saw-scaled viper (Echis c. sochureki).
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