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Functional recovery is an important aim of unicompartmental (UKA) or total (TKA) knee arthroplasty in patients with symptomatic osteoarthritis, and walking speed may be a useful variable for assessing the functional effects of knee arthroplasty.
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In one arm of the screen we used electrophysiology to assess the functional effects of the library compounds on full-length EAG1 channels.
In this study, we used recombinant Wnt3a to assess the functional effects of canonical Wnt signaling in epithelial cells and fibroblasts.
To assess the functional effects of aptamers, U87MG (300 000 cells per 3.5-cm plate) were serum starved for 2 hs and then treated with the RNA aptamers or the starting RNA G0 pool prior subjected to a short denaturation-renaturation step.
Systematic studies using both in vitro and in vivo assays to assess the functional effects of mutations that alter the sequence, but preserve (or destroy) the conserved RNA structural motif, will be required to fully evaluate its potential biological significance.
In order to assess the functional effects of different action potential (AP) kinetics in VSD imaging in cultured cells, we have simulated an 'mmature'and a 'mature' Na+-current (INa) in cultured cells using the dynamic clamp technique.
To assess the functional effects of this cleavage, we generated truncated forms of REDD1 corresponding to the cleavage products.
To further assess the functional effects of MD on these two mouse strains an echocardiography time-course was conducted.
Having established that Myc inhibition impairs autophagosome formation, we next assessed the functional effects of this on autophagy substrate accumulation.
To assess the functional effects of phospho-Akt in BO and OAC we examined the effects of stimulation or inhibition of Akt activity in OE33 cells in culture.
To assess the functional effects of miR-510 when expressed at more physiological levels, we established stable breast cell lines expressing miR-510 after lentivirus transduction.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com