Sentence examples for assessing the binding of from inspiring English sources

V-tip protein localization on the bacterial cell surface was measured by assessing the binding of a rabbit anti-PcrV IgG (1∶500) for 1 h.

Cscan compiles ChIP-seq data from published experiments assessing the binding of several transcription factors and epigenetic markers in different cell lines for human and mice.

We further investigated the effects of pH on NF-κB activity by assessing the binding of this transcription factor from nuclear extracts of mouse J774 macrophages to a consensus sequence oligonucleotide for the NF-κB promoter.

For pull-down assays assessing the binding of CaM to GST, GST-1C1b, GST-8Nt, and GST-8C2b, glutathione-Sepharose 4B supporting the appropriate immobilized GST or GST fusion protein was washed three times in PBS and added to PBS supplemented with 1% (v/v) Triton X-100, 0.5 μM His-CaM, and either 20 μM CaCl2 or 200 μM EGTA.

However, ultra-affinity was implicitely suggested in a series of works assessing the binding of different substrates to Hsp70s, always in the presence of a co-localized JDP, thus ensuring maximal hydrolysis acceleration upon substrate binding (Misselwitz et al., 1998; Laufen et al., 1999; Sullivan et al., 2001).

For comparison, we also assessed the binding of Specificity Protein 1 (Sp1), a transcription factor known to bind PCNA promoter.

Mass spectrometry (MS) is widely used to assess the binding of small molecules to proteins and their complexes.

The methodology is an inexpensive way to assess the binding of small-molecules by the stability they confer on thermal denaturation of the protein target of interest.

Measurements of thermal stability by circular dichroism (CD) spectroscopy have been widely used to assess the binding of peptides to MHC proteins, particularly within the structural immunology community.

Herein, we report a structure-activity relationship study based on the co-complex combined with bio-layer interferometry to assess the binding of our compounds.

Because the bias towards larger ligands is counter to the rules for drug bioavailability, [54] a simple metric, called ligand efficiency has been developed to assess the binding of a compound, with respect to the number of atoms, and its potential for lead optimisation [53, 55].