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In the selection step, the 'fitness' of each organism was determined by assessing the affinity of any Crx or Nrl sites in the organism's genome (scored using the PWMs and TFBS Perl module described above), the spacing between pairs of sites, whether the pairs of sites were homotypic (i.e., Crx-Crx or Nrl-Nrl) or heterotypic (i.e., Crx-Nrl) and the distance of sites from the TSS.
Alternatively, the affinity of e.g. X-13 for some specific structures in xylan may be higher than that observed when assessing the affinity for solubilized carbohydrates allowing for its use in staining of plant materials.
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As a consequence, the simple inspection of the hand or the foot is sometimes of value in assessing the affinities of animals with two, three or four digits, and this is obviously relevant when we deal with fossils.
Researchers can capitalize on these characteristics to assess the affinity and specificity of DNA-binding proteins using fluorophore-labeled oligonucleotides.
Furthermore, four HE-MBP Pyr -fused proteins were purified by immobilized metal affinity cHE-MBP Pyr -fusedassess tHE-MBP Pyr -fused with immobilized Ni2+.
The absolute value of the Dscore calculated from the docking simulation is used to assess the affinity of a peptide for t-PA.
This approach involves the use of experimental data to verify a scoring function that can be used to assess the affinity of a peptide for its target protein.
Fluorescence assays employing semisynthetic or commercial dansyl-polymyxin B have been widely employed to assess the affinity of polycations, including polymyxins, for bacterial cells and lipopolysaccharide (LPS).
So, the Dscore function is used to assess the affinity of docked peptides in a pentapeptide library designed on the basis of protein (α-amylase) structure.
The K m is used to assess the affinity of the enzyme for the substrate.
To do this, we assessed the affinity of each species towards different vegetation types.
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