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The modulation of the ERN amplitude in relation to memory decisions was interpreted as reflecting the activity of an internal monitoring device assessing the activation of the two possible decisions [29].
We here describe a method of assessing the activation of several IKs by flow cytometry.
Although therapist palpation is one recommended method of assessing the activation of multifidus [ 53], studies evaluating the reliability of this method could not be located.
When assessing the activation of both IRF-1 and NF- κB, we found the two transcription factors to be concurrently activated.
To verify the reliability of IHC in assessing the activation of the AMPK pathway, we tested the correlation between pAMPK and pACC scores on the same tumour sample, using the Spearman's coefficient.
To look specifically at the apoptotic pathway, we examined the involvement of mitochondria by assessing the activation of caspase-9 and caspase-7 which are downstream targets of this pathway.
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In addition, we assessed the activation of mitogen-activated protein kinase (MAPK) pathways.
Finally, we assessed the activation of NF-κB and activating protein-1, as well as phosphorylation of p38 mitogen-activated protein kinase by electrophoretic mobility shift assay with super shift and/or Western blot.
We initially assessed the activation of the GTPase by capturing the activated form of RalA on a GST-affinity column containing a fragment of Ral binding protein 1 (RalBP1) [19].
We assessed the activation of the STAT3 (signal transducer and activator of transcription 3) signalling pathway and the downstream targets in the cells with different expression levels of NS.
Finally, we assessed the activation of the terminal end of the signaling pathway by measuring the relative activity of a luciferase reporter under the control of the NF-κB promoter in HEK 293 cells transfected with expression vectors containing hMD-2, human TLR4 (hTLR4) and human CD14 (hCD14).
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