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Also, 622 control probes were included in the microarray for assessing synthesis variability, location of the probe within a given open reading frame and number of mismatches.
Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries.
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Therefore, we employed metabolic labelling to assess synthesis rates for FXN mRNA (Dolken et al, 2008).
A sensitive radioactive biosynthetic labeling and immunoprecipitation method was used to assess synthesis and secretion of albumin and AFP in primary fetal liver cell cultures, and after successive passage of the cells.
The present study evaluated the possibility of using cis-[18F]fluoro-proline as a non-invasive method of assessing collagen synthesis in rat musculoskeletal tissues.
Furthermore, a similar phenomenon was demonstrated in monolayer NP cell cultures using an alternative technique for assessing DNA synthesis, that is, BrdU incorporation and Table 1).
In one study, a single intra-tracheal dose of bleomycin was used and the fibrotic response determined by assessing collagen synthesis and deposition.
The toxicity of DON and its derivatives, 15-ADON and 3-ADON, were demonstrated by using the 5-bromo-2′-deoxyuridine incorporation assay assessing DNA-synthesis (Sundstøl Eriksen et al. 2004), in which there may be a link between degradation process and resistance to the mycotoxins.
To uncover whether the proliferation defect of the cultured Pold1−/− blastocysts is due to DNA synthesis inhibition caused by Polδ disruption, we assessed DNA synthesis in cultured blastocysts by the bromodeoxyuridine (BrdU) incorporation assay.
Using D2O, we simultaneously assessed protein synthesis in multiple subcellular fractions along with DNA synthesis in skeletal muscle, heart, and liver over 2 weeks in both sexes.
To assess the synthesis quality, a listening experiment was conducted with native Thai participants to identify the synthetic vowels and evaluate their naturalness.
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