In addition to assessing steady-state physiological characteristics under the three nutrient conditions, we also evaluated the temporal response of fluorescence properties to nitrogen enrichment in co-limited Synechocystis (Fig. 4).
The dynamic behaviour of the system, discussed further below, was instructive in selecting optimal dilution rates, in developing a start-up procedure for stable continuous operation and in assessing steady-state stability.
First, assuming that previous knowledge is limited and resources are insufficient to assess gene expression in a large number of discrete brain regions, we tested the effect of assessing steady-state transcription in whole brain vs. in forebrain.
While self-selected speeds are important for evaluating typical, everyday activity, future work investigating pediatric propulsion should also consider assessing steady-state target speeds since biomechanical parameters may vary with speed [ 16].
No studies were found that assessed steady state propulsion, change of direction or tilting the chair.
To expand our validation set, we identified 6 additional primary fibroblast lines (3 IPF and 3 control, which were part of the initial microarray study) that matched the sub-cultivation criteria for the original array analysis and assessed steady state protein levels for PDCD8, CFL2 and FUT10 (this requires substantially fewer cells than is needed for polyribosome preparations) (Figure 4F).
Three categories of partner type were assessed: steady (e.g., spouse, boyfriend); casual (e.g., one-night stand); and anonymous (e.g., someone in the park).
First, we assessed steady-state transcription in whole brain and two more specific brain regions.
Additional blood samples were collected at week 1 in order to assess steady-state MTX PK parameters in the presence of maraviroc.
Total RNA from human colorectal normal tissue and human CRC was prepared to assess steady-state levels of AM mRNA transcript.
To assess steady-state effects of therapeutic and supra-therapeutic doses of prucalopride on the QT interval using a novel design involving a parallel placebo group with nested crossover for positive control.
