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Our results suggest that the response to this combination treatment might depend on cells' capacities to repair DNA damage and assessing DNA repair capacities of tumor cells from patients undergoing clinical trials of PARPi could help to adjust radiation therapy schedules.
In the present study, we investigate the association of pesticide exposure and cellular DNA damage, as measured by the comet assay, which is a sensitive method of assessing DNA damage.
This ready-to-use duplex PCR assay contains an external positive control, appropriate for assessing DNA extraction efficiency and the presence of PCR inhibitors.
To compare sperm chromatin dispersion (SCD) test with terminal deoxynucleotidyle transferase-mediated terminal uridine nick-end labeling (TUNEL) assay in assessing DNA fragmentation in human sperm through bright-field microscopy.
As an independent method of assessing DNA methylation we performed COBRA analyses at the distal promoter region of Oct-4.
DNA fragmentation was also detected by comet assay which is a highly sensitive method for assessing DNA damage at the individual cell level [22].
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The toxicity of DON and its derivatives, 15-ADON and 3-ADON, were demonstrated by using the 5-bromo-2′-deoxyuridine incorporation assay assessing DNA-synthesis (Sundstøl Eriksen et al. 2004), in which there may be a link between degradation process and resistance to the mycotoxins.
When assessing DNA-PK protein expression in primary tumour biopsies from patients with cervical carcinoma, we found non-neoplastic tissue including non-neoplastic squamous epithelium and connective tissue to express no or very low DNA-PKcs, Ku86 and Ku70.
We have described two methods that use bisulfite modified DNA and Melting Curve Analysis (MCA) to assess DNA methylation.
To assess DNA replication in S15, we used BrdU incorporation assays to label newly synthesized DNA.
"Would there be a medical unit at the ready, prepared to examine blood and to assess DNA?" The upshot was a Web site, NoRace.org, and a plan for action.
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