The phrase "assessing cell surface" is correct and usable in written English.
It can be used in scientific or medical contexts, particularly when discussing the evaluation or analysis of the outer layer of cells.
Example: "The researchers are focused on assessing cell surface markers to better understand the immune response."
Alternatives: "evaluating cell surface" or "analyzing cell surface".
We assessed cell surface marker expression on monocytes and granulocytes for HLA-DR and toll-like receptor (TLR -2 and TLR -2
To assess cell surface hydrophobicity [67], stationary phase bacteria were inactivated in 0.5% NaN3 at 37°C overnight, washed twice with 97 mM K2HPO4, 53 mM KH2PO4, 0.8 mM MgSO4, 21 mM urea, and adjusted to an OD470 of 1.0.
The authors assessed cell surface receptors to distinguish between conventionally activated T cells and T cells that expand homeostatically.
We also assessed cell surface expression of mesothelin by flow cytometry in early and late passage cells (Additional file 2).
Flow cytometry and mammosphere assays were used to assess cell surface markers and stem cell-like properties, respectively.
We again treated cells to the same hypoxic and normoxic conditions and assessed cell surface expression of CXCR4 by FACScan analysis.
Given the current lack of antibodies directed at the extracellular domain to assess cell surface expression of BMPR-II, we employed an alternative method.
Cells were equilibrated with annexin VFITC or annexin VCY5 (Becton Dickinson) to assess cell surface PS exposure, or with propidium iodide for 4 min to assess cell death, and were analysed by flow cytometry on a FACScalibur machine using CellQuest (Becton Dickinson) or Flowjo (Tree Star, Ashland, OR, USA) software.
We also assessed cell surface α5 integrin sub-unit by flow cytometry analysis of non-permeabilized cells and found increased fluorescence intensity for the DDR1 over-expressing cells (DDR1 OE - 2.2±0.3 fluorescence channel number; DDR1 wild type - 0.6±0.1 fluorescence channel number; P<0.05).
As a result, we initially used the biosensor to assess cell surface-associated furin in fibrosarcoma HT1080, breast carcinoma MCF-7, and glioma TP98G, U373 and U251 cells, which naturally express different levels of furin and also in colon carcinoma LoVo cells.
