Further afield, derivatives of the "quorum-sensing" circuits of bacteria, that assess cell density to regulate interconversion between biofilms and other growth forms [ 15], and components of the plant phytochrome system, in which promoter activity can be regulated by light [ 16], have been adapted for artificial promoter construction.
To assess cell viability, optical density (OD) was measured at 490 nm with an enzyme-linked immunosorbent assay (ELISA) plate reader.
(A,B,E,F,I,J, M,N,Q,R,U,V) HE staining; (C,G,K,N,R,V) Iba1 immunohistochemistry to assess microglial cell density and morphology; (D,H,L,P,T,X) GFAP immunohistochemistry to assess astrocyte density and morphology.
To assess endothelial cell density (ECD) and model 10-year cell loss after Descemet stripping endothelial keratoplasty (DSEK), and to compare with results after penetrating keratoplasty (PK) performed for the same indications in the Cornea Donor Study CDSS).
In bacteria, this cooperative behavior is typically regulated by quorum sensing (QS), a chemical communication system in which cells produce diffusible molecules and can assess the cell density by sensing the local concentration of these signaling molecules [11], [12], [22], [23].
Diffusion-weighted imaging can assess tumor cell density and differentiate brain tumors from brain abscesses.
Our results suggest a quantitative utilization of these data to assess tumor cell density in cervical cancer samples.
Despite the fact that many different image processing systems could be used, we here implemented the procedure for assessing immune cell densities in cell conglomerates using the Visiomorph software (VisioMorph, Visiopharm, Denmark).
To assess cell growth, ESCC cells were plated at a density of 20,000/ml of culture medium.
To assess the corneal endothelial cell density (ECD) in keratoconus patients with no history of contact lens use.
