Sentence examples for assessing binding to from inspiring English sources

We concluded that this FP assay was reliable for assessing binding to EHD1-EH.

A direct interaction was next explored by assessing binding to the ENTH domain of epsin 1 (with an His tag at the C-terminus) to increasing concentrations of the cytosolic portion of Syb2 fused to GST.

To assess binding to the native conformation, conditions that readily denature myosin, e.g. dilute protein concentrations at elevated temperatures, were avoided.

However, we wished to assess binding to combinations of sphingolipids and gangliosides in a more membrane-like setting that would support microdomain formation.

Accordingly we employed recombinant BSP, BSP purified from bone, or recombinant human BSP to assess binding to proMMP-2.

To address if QIL1 also interacted with MIC10, we immunopurified endogenous MIC10 in isolated mitochondria from 293T cells and assessed binding to QIL1 by western blot analysis.

Radioligand binding assays were contracted to CEREP (Celle l'Evescault, France) where membranes from CHO-cells expressing either hCB1R or hCB2R were used to assess binding to the two cannabinoid receptors.

Here, we present detailed instructions for assessing binding stoichiometry and affinity to assembled unstabilized microtubules using radiolabeled drug.

To test this hypothesis, we performed ChIP to assess binding of ESE3 to the promoters in parental and ERG-expressing LNCaP cells.

To test this hypothesis, we performed ChIP to assess binding of ESE3 to the regions of the EZH2 and Nkx3.1 promoters containing the identified EBS in parental and ESE3-kd LNCaP cells.

To assess binding of KatA to monomeric or polymeric Vn, 50 nM Vn was coated to Polysorb plates as described above.