Sentence examples for assessing binding of from inspiring English sources

For assessing binding of lymphoid-specific transcription factors in Figure  5G, peak calling was performed by using the Hypergeometric Optimization of Motif EnRichment (HOMER) tool (v4.1) [ 76] with default settings (FDR: 0.001; local and input fold enrichment: 4.0).

Since TESS predicted that Sp1 binds to MCRE, we assessed binding of cellular Sp1 to MCRE by electrophoretic mobility shift assay (EMSA).

To assess binding of KatA to monomeric or polymeric Vn, 50 nM Vn was coated to Polysorb plates as described above.

Two mechanisms were assessed: binding of the tracer to melanin or to sigma receptors of melanoma cells.

Next we assessed binding of recombinant SNAP25 and VAMP2 to synatxin1B immunopurified from mouse brains.

To confirm the DNA-binding data, we employed chromatin immunoprecipitation (ChIP) assays to assess binding of Stat1 and Stat3 to the Cdkn1b promoter in vivo (Fig. 3B).

To assess binding of CPS to LL-37 in the presence of human CD14 and LBP, an EMSA assay using native gel conditions was performed [18].

To assess binding of recombinant HcpA for CFH and CFHR-1, ligand affinity blotting techniques and ELISA were employed in combination with intact HcpA and various deletions thereof.

To test this hypothesis, we performed ChIP to assess binding of ESE3 to the promoters in parental and ERG-expressing LNCaP cells.

To validate the binding of NLX to FLNA, we assessed binding of [3H]NLX to membranes prepared from the human melanoma cell line M2 that lacks filamin and to membranes from its FLNA-transfected subclone A7.

As opposed to Type I pheromones that gave very low background indicating negligible non-specific binding (see buffer in Fig. 12), it was difficult to assess binding of the pentaene compounds because their hydrophobicity led to high background levels.