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This was accomplished by assessing activation of cognate and noncognate promoters from the traAFBH operon by alleles of traR from different sources.
We also determined if PF-228 perturbed the GPVI-FcRγ signaling axis by assessing activation of the tyrosine kinase Syk using anti-Syk pTyr 525/526 antibody.
In order to better select patients that will respond to targeted treatment Dry and colleagues employed a pathway-based approach to derive a gene expression signature predictive of sensitivity to MEK inhibition by assessing activation of MEK and compensatory signaling from other RAS effectors [ 15].
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To test whether between-group differences in processing the neutral conditions might have influenced our results, the contrast assessing activations of neutral images was also defined.
To identify the key functional pathways downstream of activated JAK2, we assessed activation of Stat1, 3, and 5, Pi3k/Akt, and Mek/Erk in the presence and absence of TG101209.
A specific caspase-inhibitor CaspACE FITC VAD FMK In Situ Marker (Promega, Mannheim, Germany) was used to assess activation of Caspase-like cysteine protease activity in the parasite, following manufacturer's instructions.
To confirm apoptosis, we assessed activation of cellular caspase 3 using FITC-conjugated active caspase 3 antibody, followed by FACS analysis.
Scintigraphy using 99mTc-3PRGD2 targetintegringrin αvβ3 could assess activation of hepatic stellate cells (HSCs).
Many questions remain regarding the use of TSPO tracers as diagnostic tools to assess activation of microglia.
The morphological change of the adherent platelets is a common qualitative criterion to assess activation of adherent platelet on the materials' surface.
To assess activation of muscles of hip adduction using EMG and force analysis during standard clinical tests, and compare athletes with and without a prior history of groin pain.
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