To assess whether modifications in the diaphragm are responsible for the impairment in respiratory function in DMSXL mice, we first examined diaphragm sections stained for either ATPase activity or with hematoxylin and eosin (H&E).
To assess whether modifications of the algorithm would increase the PPV without decreasing the sensitivity, additional analyses were performed.
To assess whether modification at K127 was mediated solely by Ubc9 or depended on a second E3, we deleted SIZ2 in addition to SIZ1, and now conjugation was completely abolished.
G.J. and A. B. assessed tRNA modifications in elp mutants.
Further work is needed to assess whether these modifications modify the catalytic activity of USP25.
We assessed whether histone modifications by methylation or acetylation influence the observed training effects of MDP and flagellin.
The goal of this pilot study was to assess whether dietary modifications, designed to decrease dietary omega-6 fatty acids and increase omega-3 fatty acids, influence the expression of genes that are involved in inflammation in patients with CDH.
To assess whether these modifications might regulate Che-1 dissociation from p53, we performed co-immunoprecipitation assays using p53-null EJ cells overexpressing p53 WT or a p53 mutant lacking only serine 46 (S46a), a phosphorylation target involved in apoptosis induction.
Since the identification of a cyclin partner would allow us to assess whether Lsk1p activity was regulated by modifications in cyclin stability and/or localization we decided to determine whether such a physiologically relevant cyclin sub-unit existed in S. pombe.
To assess whether the modification of UCP2 activity observed in patients 1 and 2 could affect insulin secretion in beta cells, we subjected an insulin-secreting pancreatic insulinoma cell line (INS-1E) [35] to adenoviral expression of wild type and mutated UCP2 in mitochondria.
As O-GlcNAcylation of FoxO1 has been shown to increase its transcriptional activity23, we assessed whether this modification of FoxO1 plays a role in Ang-2 upregulation upon NDPK-B depletion.
