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Currently, two methods of determining HER2 status are used routinely in clinical practice: immunohistochemistry (IHC), which assesses expression of the HER2 protein on the cell surface, and fluorescent in situ hybridisation (FISH), which detects amplification of the HER2 gene (Pauletti et al, 2000; Bartlett et al, 2001; Lebeau et al, 2001).
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To assess expression of antigenic markers by mTert-GFP-expressing cardiac cells, hematopoietic cells were excluded from the analysis.
We first assessed expression of 10 genes identified by Suarez et al. to be differentially expressed comparing bacterial to viral infections.
The present study was designed to assess expression of iNOS in AAA in human beings.
Our objective was to assess expression of key constituents of these pathways, in alliance with contractile function, through late gestation and during term and preterm labor.
We evaluate the batch effect after scaling normalization by assessing expression of commonly used housekeeping genes, including Actb, Actg1, B2m, Rps6, and Rpl13 (all of these genes are expressed in >20% of cells in each batch) and confirmed that our normalization procedure reduced differences between batches (Supplementary Figure 23c).
(A) Semi-quantitative real-time PCR to assess expression of pro-inflammatory and pro-fibrotic cytokines, IL-1β, TNFα, iNOS and tgf-β1, in the lungs of sham and BLM-treated WT and Ninj1 KO mice at day 3, 7, 14 and 21 (n = 3).
It was not possible to assess expression of Glut1.
Real-time polymerase chain reaction (PCR) was used to assess expression of MMP-3 and MMP-9 mRNA.
Since the most important TGFβ isoform in humans is represented by TGFβ1, we assessed expression of this particular molecule in our study.
In this study, we used a qPCR platform (previously described) to assess expression of 188 microRNAs.
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