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The colorimetric CCK-8 assay assesses cell viability based on the ability of living cells to reduce soluble WST-8 to formazan.
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To assess cell viability, treated cells were subjected to a quantification of nuclear fragmentation or ATP content.
The MTT (3- 4, 5-dimethylthiazolyl-2)−2,5-diphenyltetrazolium bromide) assay was used in order to assess cell viability and cytotoxicity.
The MTT assay was employed to assess cell viability at Day 2.5 and Day 4.5 after cells were loaded on the sericin hydrogels in the cell cullture dishes.
In experiments assessing cell viability, after treatment cells were detached using trypsin-EDTA (ThermoFisher), diluted into trypan blue, and then percentage of stained cells analyzed using an automated cell counter (TC20, Bio-Rad).
After cells had been resuspended in 1 ml fresh culture medium, 10 μl of the cell suspension was mixed with an equal volume of 0.4% Trypan blue dye (Bio-Rad) to assess cell viability.
To assess cell viability, after 24 h incubation with the different treatments, 5 µL of resazurin (500 µM, Sigma Aldrich) were added to each well leading to a resazurin final concentration of 50 µM.
Wild type HIV and envelope-minus HIV pseudotyped with Vesicular Stomatitis Virus glycoprotein (VSVg) were used as controls to assess cell viability after inhibiting clathrin pathway.
We compared FCXM with its modified version assessing cell viability (cytolytic flow cytometry crossmatch; cFCXM) using sera from previously sensitised kidney recipients.
Positron emission tomography (PET) imaging was used to assess cell viability non-destructively over periods extending up to a few weeks.
Fluorescein diacetate (FDA) was used to visually assess cell viability (Widholm 1972).
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