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Hence, penetration through Matrigel assesses cell migration rather than cell invasion.
The lactate dehydrogenase (LDH) assay (CytoTox-One; Promega) assesses cell death by measuring LDH that leaks out of dead cells into the media.
The colorimetric CCK-8 assay assesses cell viability based on the ability of living cells to reduce soluble WST-8 to formazan.
We devised a semi-quantitative measure of neuronal damage that assesses cell death across brain regions as follows: brains were divided into three regions (hippocampus, cortex and striatum) and scored using the following criteria: 0, no neuronal damage; 1, slight histological abnormalities; 2, focal areas of neuronal loss; and 3, majority of neurons in the region lost.
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assessing cell growth in damaged tissue; 6.
To assess cell death in intratesticular grafts.
MTT assay was used to assess cell growth.
Immunohistochemistry was performed in order to assess cell adhesion and neurite extension.
Fluorescein diacetate (FDA) was used to visually assess cell viability (Widholm 1972).
To assess cell attachment and growth on the scaffold, human mesenchymal stem cells were cultured on the scaffolds.
HEK 293T cell was selected in the present study to assess cell viability and spreading on aligned CNF.
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