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The specific surface area was assessed utilizing the Brunauer Emmett Teller (BET) technique, and the pore size distribution was estimated from desorption branches of nitrogen isotherms using the Barrett Joyner–Halenda (BJH) system.

Deconvolved images were assessed utilizing the ImarisColoc software (Bitplane) in manual mode.

Viability was assessed utilizing the Live/Dead assay kit (Molecular Probes).

Oxidative stress was evaluated using Malondialdehyde (MDA) equivalents which were assessed utilizing the thiobarbituric acid (TBARS) as reactive substrate [ 35].

The statistical heterogeneity among the RCTs selected for meta-analysis was assessed utilizing the DerSimonian Laird estimate τ for inter study variance.

Missense mutations were highlighted if they occurred within highly conserved residues in vertebrates, assessed utilizing the UCSC Genome Browser (http://genome.ucsc.edu/) conservation track tool [ 17].

Response to therapy was assessed utilizing an adaptation of the International Uniform Response Criteria for Multiple Myeloma (Table  3) [ 15].

Anxiety and depression were assessed utilizing HADS [16].

We also assessed proliferation utilizing the marker Ki67.

Alternatively, surgical resection margins on the BCS specimen can be assessed by utilizing the so called "tangential shaved margins" technique [ 9, 55, 59, 81].

Conversely, we realize that gentle pressure is subject to interpretation and even more difficult to assess if utilizing the probe through an endoscope.