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The number of CA1 uninjured neurons was assessed utilizing a stereological approach.
The level of active Raf-bound Ras was assessed utilizing a Ras activation assay kit (Upstate, Lake Placid, NY).
The recovery cycle of excitability was assessed utilizing a paired pulse protocol, with a supramaximal stimulus followed by a test stimulus at intervals varying between 2.5 and 200 ms. Initially following conduction of an impulse, it becomes more difficult to generate a subsequent impulse due to the recovery of inactivation of transient Na+ channels [26], [27].
PLD activity was assessed utilizing a PLD transphosphatidylation reaction unique to this enzyme.
Nutritional status was assessed utilizing a combination of SGA and serum proteins measurements (serum albumin, serum prealbumin).
At 24, 72, and 120 h, cell proliferation was assessed utilizing a nonradioactive cell proliferation assay (Promega, Madison, WI) according to the manufacturer's protocol.
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Volatile organic ingredients of the cleaning mixtures were assessed utilizing an integrated sampling and analytic method, EPA TO-17.
Response to therapy was assessed utilizing an adaptation of the International Uniform Response Criteria for Multiple Myeloma (Table 3) [ 15].
This was performed as described in 2 cases [ 20], and using a modification of the published protocol in 1 case, methods for which are given in Additional file 1. Response to therapy was assessed utilizing an adaptation of the International Uniform Response Criteria for Multiple Myeloma (Table 3) [ 15].
Finally, it is non-trivial to integrate information from multiple sources in a unified manner such that coherence of the gene subset is assessed utilizing all information.
This will be assessed utilizing the Herth Hope Index [ 21, 22], a validated instrument developed for use with the terminally ill.
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