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The expression of apoptotic, ER stress-related and signaling proteins were assessed using Western blot analyses.
eNOS protein expression in reperfused heart was assessed using Western blot method.
Levels of AMPK, Akt JNK, ACC, SERCA, membrane Glut4, AS160 and PGC-1α were assessed using Western blot.
The effect of transferrin on stellate cell activation was assessed using western blot analysis for α-smooth muscle actin expression, [3H]Thymidine incorporation, and real-time RT-PCR for procollagen α1(I) mRNA expression.
PINK1 overexpression lines had equivalent expression of protein, assessed using western blotting (Fig. 1A).
Stress signaling and Ca2+ regulatory proteins were assessed using Western blot analysis.
Similar(21)
Then, the expression of four apoptotic factors; Bax, Bcl2, caspase3 and PARP, in the NAc and PFC were assessed using the Western blot technique.
Preoperative clinical status was assessed using the Western Ontario McMaster Universities Osteoarthritis Index (WOMAC) scales [14].
Furthermore, the injury-related quality of life was assessed using the Western Ontario Shoulder Index (WOSI).
Knee pain was assessed using the Western Ontario and McMaster Universities Osteoarthritis Index.
Symptomatology and disability was assessed using the Western Ontario and McMaster Osteoarthritis Index (WOMAC) questionnaire [ 47].
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