Library quality was assessed using the Agilent DNA High-Sensitivity Bioanalyzer kit, and library quantity was assessed using the High-Sensitivity Qubit Fluorometer (Invitrogen).
bicolor juice samples for which brix had been measured were assessed using the high-throughput FTIR-based PLS models.
The quality and size (~260 bp) of the resulting cDNA libraries were assessed using the High sensitivity DNA Kit (Agilent Technologies, Inc). in an Agilent Bioanalyzer 2100 (Agilent Technologies, Inc).
A block randomization scheme is employed, balancing gender and baseline impact of modifiable cardiovascular risk factors assessed using the INTERHEART Modifiable Risk Score (highest tertile vs. middle tertile).
Tumor budding was assessed using the 10 high-power fields (10HPF) method (40×; HPF field area 0.049 mm) of highest density along the invasive front [ 25, 26].
Libraries were quantified by qPCR using the KAPA Library Quantification Kit for Illumina Libraries (KapaBiosystems, Wilmington, MA) and library profiles were assessed using the DNA High Sensitivity LabChip kit on an Agilent Bioanalyzer.
Socio-economic status was assessed using the following variables: highest level of education, classified as no education, primary, secondary or higher.
The potential ability of the CDS-ID, when used as a covariate, to reduce the confounding effects of comorbidity was assessed using the relationship between high-risk antibiotic exposure and CDI as an illustrative example.
