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Cell attachment and proliferation on solvent-cast films was compared with commercially available wound dressings while cell attachment, proliferation and viability on biaxially stretched films were assessed using light, confocal laser and scanning electron microscopy (CLM and SEM).
Platelet reactivity was assessed using light transmission aggregometry and P-selectin, and glycoprotein IIb/IIIa receptor expression was assessed using flow cytometry.
The number of newly generated osteoclasts and the number of nuclei per osteoclast (after counterstaining with DAPI) were assessed using light microscopic examination.
Cell migration from the spheroids was assessed using light microscopy.
Peribronchial and perivascular inflammation was assessed using light microscopy under × 50 magnifications.
Cell viability following harvest was assessed using light microscopy and Trypan blue dye exclusion.
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Lung deposition during pressure support ventilation was nonetheless substantial and should be further assessed using lighter sedation.
Although measurement of the OD has provided objective quantitative data on the amount of hsp-27 expressed, which is recognised to be difficult to assess using light microscopy, its precise significance remains to be established.
Surface morphology of the retrievals was assessed using white light interferometry.
CLs and miRNA-loaded cationic liposomes (CL/miR-101) were prepared and their characteristics were assessed using Dynamic light scattering (DLS) technique.
During cell culture, in situ imaging and morphological characterisation of cells was assessed using brightfield light and/or fluorescence microscopy, and later confirmed by staining of fixed cells using immunofluorescence microscopy.
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