injection of glutamate, N-methyl-d-aspartate (NMDA), bradykinin (BK) and prostaglandin E2 (PGE2), and the nociceptive response was assessed using hot-plate test.
Table 1 shows the antinociceptive effect of HMCR and standard drug (Morphine) assessed using the hot plate test.
Multiple sequence alignment quality was assessed using the HoT-method (Landan and Graur 2007).
Nociceptive behavior was assessed using paw pressure, hot tail immersion, and formalin tests.
Heat hyperalgesia and allodynia of the hind paw were assessed using Eddy's hot plate as described method of Eddy et al. [ 43], for assessing the reactivity to noxious and non-noxious thermal stimuli respectively.
Similarly, the association between all factors and the severity of hot flashes was assessed using proportional-odds ordinal regressions.
The association between reported hot flash frequency and both primary and secondary factors was assessed using over-dispersed Poisson models, both unadjusted (univariable) and multivariable regressions.
Metabolic response was assessed using 18FDG-PET/CT according to the PET response criteria in solid tumors (PERCIST) v1.0 guideline, comparing SUVmax of the hottest single lesion in each consecutive scan (metabolic response definition: reduction in SUVmax of ≥30%).
Subjective sleepiness was assessed using the Stanford Sleepiness Scale.
Time trends were assessed using birth cohorts.
