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Quality control was assessed using custom Perl [ http://www.perl.org/] scripts (available from the authors on request).
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Locomotor activity levels were recorded with a webcam, and assessed using custom-written software (MATLAB vR2007a, MathWorks, MA, USA) that detected thresholded pixel differences for pairs of consecutive frames and then calculated the summed activity scores for each 5-s epoch over the 4-h recording duration (2880 epochs per rat).
Hypermethylation of the BRCA1 promoter was assessed using a custom methylation specific MLPA set according to the manufacturer's protocol (ME005-custom; MRC-Holland, Amsterdam, the Netherlands).
Genetic diversity among rice cultivars from Bangladesh and North East India was assessed using a custom 384-SNP microarray assay.
Parameters such as cooling rate, plunge temperature and cryoprotective agent concentration were assessed using a custom built device based on Pye's freezer idea.
The statistical significance of the difference in the AUC values between two genetic interaction measures was assessed using a custom written algorithm based on the method of DeLong et al. [30].
The 6-day cytokine RNA levels were also assessed using the custom TLDA.
The expression levels of the PRDM genes were assessed using a custom TaqMan® Low Density Array (Applied Biosystems, Foster City, Cal., USA).
Global expression of Wnt signalling and target genes in response to TNFα and glucocorticoids was assessed using a custom array.
Twenty-three genes in the panel were assessed using a custom microarray comparative genomic hybridization (CGH) chip (Agilent Technologies, Santa Clara, California).
The expression of Rapamycin regulated genes in the brain was assessed using Agilent Custom arrays followed by Significance Analysis of Microarrays (SAM) analysis of the 1165 identified rapamycin-regulated genes only.
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