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Dietary adherence was assessed using capillary ketone levels (Xceed Optium; Abbott Diabetes Care, Maidenhead, UK).
After more than 1 year of storage at -80°C, the total RNA quality was assessed using capillary electrophoresis.
Native protein AKT profiles assessed using capillary tube isoelectric point focusing are sensitive to phosphorylation events [ 27].
RNA integrity was assessed using capillary gel electrophoresis (Agilent BioAnalyzer, Agilent Technologies, Palo Alto, CA) to determine the ratio of 28s:18s rRNA in each sample.
PPP was assessed using capillary electrophoresis, which separates the protein components into five major fractions by size and electrical charge: prealbumins, albumins, α-1 globulins, α-2 globulins, α-3 globulins, β-1 globulins, β-2 globulins and γ globulins.
RNA integrity was assessed using capillary gel electrophoresis (Agilent 2100 Bioanalyzer; Agilent Technologies, Inc., Palo Alto, CA, USA) to determine the ratio of 28s:18s rRNA in each sample.
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The influence of drugs on feeding was assessed using the capillary feeding assay [50].
Feeding behavior was assessed using a capillary feeding assay described previously [ 6].
HbA1c levels will be assessed using a capillary test and analysed using the DCA 2000 apparatus (Siemens Medical Solution Diagnostics, Mölndal, Sweden).
RNA quality and quantity were assessed using automated capillary gel electrophoresis on a Bioanalyzer 2100 with RNA 6000 Nano Labchips according to manufacturer's instructions (Agilent Technologies Ireland, Dublin, Ireland).
RNA quality and quantity were assessed using automated capillary gel electrophoresis on a Bioanalyzer 2100 with RNA 6000 Nano Lab-chips according to manufacturer's instructions (Agilent Technologies Ireland, Dublin, Ireland).
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