The optical density of the color reaction mixture was assessed using an automatic on a multiwall scanning spectrophotometer (Biotek, Winooski, VT) at 450-nm absorbance.
Serum concentrations of glucose, TG, and HDL cholesterol were assessed using an automatic analyzer (RA 1000, Technicon Corporation, Tarrytown, NY, USA) and a commercially available kit.
Systolic and diastolic blood pressures were assessed using an OMRON HEM-907XL automatic inflation sphygmomanometer, which has been used in large scale clinical trials [ 28, 29].
The ML nodal support was assessed using the fast bootstrapping algorithm via automatic estimation of runs in RAxML [32] as implemented in the CIPRES portal v.1.15 (2010) http://www.phylo.org/.
Effects of intensive language action therapy (ILAT) on automatic language processing were assessed using Magnetoencephalography (MEG).
Inflammatory and calcification markers were assessed using ELISA micro-plate immunoassays and ELX808 automatic reader (BIO-TEK® Instruments Inc., VTrmont, VT, USA).
Motor functions were assessed using a rotarod apparatus and a CatWalk gait automatic analyzer.
CIMT was assessed using high-resolution B-mode ultrasound with a 10-MHz linear transducer, with an automatic boundary detection system (Art Lab-Esaote-Picus, Genova, Italy).
The baPWV was assessed using VP-1000 vascular profiler (Nippon Colin Ltd., Komaki City, Japan), which allowed on-line pulse wave recording and automatic calculation of PWV.
The concentrations of CEA and CYFRA21-1 in the serum were assessed using electrochemiluminescence immunoassay (ECLIA) kits (CEA, lot: 172356; CYFRA21-1, lot: 169393; Roche, German) on a Roche E170 fully automatic electrochemistry luminescence immunity analyzer (Roche, German).
