b Inner membrane permeability was assessed using ONPG absorbance after expression had been induced at 25 °C for 60 h and 72 h, or at 25 30 °C (shifting from 25 to 30 °C at 32 h) for 60 h and 72 h, respectively.
The quality of the isolated DNA was assessed using the absorbance 260/280 ratio and 1% agarose gel electrophoresis.
Disaggregation was assessed using turbidity (absorbance at 395 nm) (Couthouis et al., 2012; Couthouis et al., 2011; Sun et al., 2011).
Humic substance co-extraction was assessed using the absorbance ratio A260/230, whereas the ratio A260/280 indicated co-extraction of proteins and high amounts of RNA.
Humic substance and protein co-extraction was assessed using the absorbance ratio A260/230, whereas the ratio A260/280 indicated co-extraction of proteins and high amounts of RNA.
HRP activity was assessed using 3,3′,5,5′-tetramethylbenzamidine 3,3′,5,5′-tetramethylbenzamidinem.
Another key issue is stability under thermal stress, which can also be assessed using the RC absorbance spectrum.
The quality was assessed using the ratio of absorbance at 260 nm to 280 nm (A260/A280) and by running on denaturing 1% agarose gel to confirm the presence of non-degraded RNA.
Cell viability is assessed using two separate assays – absorbance-based XTT, which measures mitochondrial enzyme activity, and fluorescence-based CellTiter-Blue, which measures mitochondrial, cytoplasmic and microsomal enzyme activity.
Absorbance was assessed using Chameleon 5025 (HVD Life Sciences, Vienna, Austria).
