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The deacetylase potential of SIRT1-ΔExon8 wassessedsed using a standard fluorometric in vitro deacetylase assay (see: Methods).
Predictions based on different docking methods were assessed using a standard set of statistical indicators to evaluate different ligand and docking classification approaches: sensitivity, specificity, overall prediction accuracy, and Matthews correlation coefficient (Kortagere et al. 2009).
Heterogeneity was assessed using a standard Q- test.
Weight was assessed using a standard scale.
Study quality was assessed using a standard grading scale.
To assess the significance level of indirect effects, the standard error of estimated parameters of indirect effects was assessed using a bootstrap method (1000 iterations) (Rosseel 2012; Zainuddin 2015).
Stomach barrier function was assessed using a FITC-labelled dextran method.
Haemoglobin will be assessed using a portable spectrophotometer (HemoCue AB, Angelholm, Sweden) for controls or using standard hospital laboratory methods for cases.
RNA samples were first assessed using a NanoDrop™ spectrophotometer and an Agilent bioanalyzer and then subjected to a 2-color standard Agilent amplification labeling method.
Gastro-oesophageal reflux will be assessed using the gold standard method, 24-hour pH-metry [ 49].
During each exposure to the NEC and ALN conditions behavior was assessed using standard methods by trained raters using a closed circuit television system [6].
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