Here we reported the characterization of four key C4 pathway genes and assessed their expression patterns and enzymatic activities at three growth stages in flag leaves of 59 bread wheat genotypes.
To further investigate these proteins, and how they may relate to long-term stress exposure, we assessed their expression patterns in young versus aged mammalian cortical tissue from multiple sources, i.e. rat, rhesus macaque and human.
It has to be noted that this verification method fails for factors such as SRF and HNF1, which are broadly expressed despite their known tissue-specific activities, or for factors such as PTF1, which do not have enough support by EST data to assess their expression patterns.
Transcription factors with enriched binding site predictions were additionally assessed for their expression pattern at E13.5, 15.5, and 18.5 using images from the Allen Mouse Brain Atlas (ABA, http://www.brain-map.org/) [ 69].
To address this, 10 of these transcripts that showed inconsistent results from RNA-Seq and DGE platforms were randomly selected to assess their relative expression patterns among CK, CA1 and CA3 using quantitative RT-PCR approach (qRT-PCT).
A possible role of GRAS genes in the loss of rooting capacity was analyzed by assessing their temporal expression patterns in response to auxin in hypocotyl and epicotyl cuttings from 21- and 90-day-old seedlings.
As Egr1 and Egr2 are immediate-early genes induced by a wide variety of stimuli, their expression pattern was assessed in 3T3-L1 cells following addition of differentiation cocktail (MDI: iso-butyl Methylxanthine, Dexamethasone and Insulin) (Fig. 1a).
Nine of the differentially expressed genes identified from the microarray analysis were further assessed by qRT-PCR to validate their expression pattern (gene names and primer sequences are given in Table S10).
Due to increasing awareness of the CP as fundamental to the development of CNS inflammation [ 4- 6], immuno-LCM coupled to qrt-PCR array was used to separately acquire CP stromal capillary and choroidal epithelial tissues and assess their respective patterns of expression in situ of a wide panorama of immune-related genes.
Next, we assessed the expression patterns of differentiation marker genes in order to understand when during the neuronal differentiation process their individual gene expression variance and co-expression relationship with associating genes changed.
We assessed gene expression patterns by qPCR, global DNA methylation by ELISA, and proteome lysine acetylation status by Western blot in cardiac tissue from saline and DOX-treated rats.
