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To understand role of BbCRASP-2 in B. burgdorferi infectivity, we first assessed the transcript levels of BbCRASP-2 in multiple murine tissue locations where B. burgdorferi persists during infection, and in various stages of infected ticks.
To identify the role of PAP5 in the resistance against B. cinerea, we assessed the transcript abundance of PR1 and PDF1.2.
Hence, we next assessed the transcript levels of SREBP2 and SREBP1 in HepG2, MCF7 and HEK293T cells after overexpression of p(128).
We assessed the transcript abundance for each gene and converted raw read counts to RPKM (reads per kilobase per million mapped reads).
We then assessed the transcript activation in MSCs co-cultured with activated TIL by IPA by using the list of genes differentially expressed between MSCs co-cultured with TIL+M38 and the MSC control group (P value and FDR <0.05).
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To assess the transcript knockdown, total RNA (Qiagen) was extracted from the dissected tissues (head, abdomen, midgut and ovary) of ten dsRNA injected females.
To comparatively assess the transcript levels of LEC1 in the embryo and endosperm of B. napus, we performed RT-PCR with RNA samples from endosperm and embryos at the cotyledon stage.
To assess the transcript profile changes in calreticulin-truncated mutants, samples were analyzed using Genespring GX to visualize expression dynamics and extract significant differences in each of the truncation mutants compared to wild type control embryonic stem cells.
Semi-quantitative RT-PCR analyses at least in triplicates were performed to assess the transcript regulation of selected genes in leaves 6 h after fumigation beginning with the respective CO2 concentration (Fig. 3).
While expression array analysis of each cross therefore does not assess the transcript abundance variation associated with a single genotype, assay of these crosses provides a reasonable estimate of the variation observed in this D. simulans population.
To further assess the transcript coverage and to estimate how the coverage depth affected the assembly of the Unigenes, the reciprocal TBLASTX was performed, and the correlation between the ratios of the assembled UniGene lengths to the lengths of Spruce orthologs and coverage depth were surveyed using a scatter plot.
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