Sentence examples for assessed the immunostaining from inspiring English sources
ZZ assessed the immunostaining.
HY and ZZ assessed the immunostaining.
Two experienced pathologists assessed the immunostaining in a blinded fashion as described previously (Cheng et al, 2008).
Numbers of PSA-NCAM immunopositive cells were quantified [26] using the software program Neurolucida (MicroBrightfield Inc, Williston, VT, USA), and 5-HT1A immunoreactivity was quantified using Image J, a public domain image processing program (http://rsb.info.nih.gov/ij/) that assessed the intensity of immunostaining [25].
The pathologists assessed the adequacy of immunostaining in test slides and in negative and positive control slides, and then selected representative areas of tumour for counting.
One further study has assessed the pattern of immunostaining of hsp-27 (also called 24-kDa protein) in a range of benign breast biopsy specimens and showed little or no staining in ducto-lobular tissue or fibroadenoma, but high levels of staining in apocrine metaplasia (Courtney et al, 1990).
To ensure that we did not overlook subtle effects on cell cycle regulation/proliferation, in addition to the BrdU incorporation assay, which measures the percentage of cells in S-phase, we also assessed the proliferative index by immunostaining for phospho-histone H3, a marker for cells in M-phase.
Next, we assessed the mTOR activity using phospho-S6 immunostaining.
To determine whether this inhibition of angiogenesis is also present in p190B+/-Neu tumors, we assessed the number of vessels using immunostaining for an endothelial specific marker (VWF).
We then assessed the microvascular density (MVD) by immunostaining endothelial cells with an anti‐CD31 antibody and calculated MVD as a percentage of tumor section area using the Chalkley grid counting method.
To assess the reproducibility of the assessed surface immunostaining phenotypes, we have repeated the measurement of each assessed immune phenotype (with the exception of CD19+CD5+ B cells) using an independent PBMC sample from 18 donors, within 1 2 months of the original measurement.
