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To test this idea, we assessed the assembly of TCR signalosome in thymocytes from BALB/c c-FLIPL Tg mice and their NLC.
We assessed the assembly quality using bacterial artificial chromosome (BAC) clones and found that the scaffolds were reliably assembled with the exclusion of the GC-rich and repeat-rich regions, which were filled by gaps (Supplementary information, Figure S1).
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Aiming to develop and grant maturity milestones, standardized procedures are used to assess the assembly reliability.
To assess the assembly quality, we used the N50 statistic.
Therefore, to assess the assembly quality as a function of metagenome's complexity, we constructed three datasets using the profiles described in [ 5].
In order to assess the assembly quality, the transcriptome was sequenced and aligned to the scaffold sequences using Blat with default parameters [ 46].
To assess the assembly quality, all read sets were realigned on the contigs and had an alignment rate of at least 80%.
Next, we used the T. castaneum set of Benchmarking sets of Universal Single-Copy Orthologs (BUSCOs; 2,787 genes) to further assess the assembly completeness (Waterhouse et al. 2013).
This strain is a duplicate of L. monocytogenes F2365 and was included to assess the assembly methods used in this study.
To further assess the assembly, we compared the contigs plus singletons (hereafter referred to as Torvum unigenes) against selected public assemblies, including the recently released 6,296 unigene catalogue from Solanum torvum cv.
Since several full-length coding sequences of buckwheat genes were available in GenBank, we used this information to assess the assembly quality by mapping the contigs onto these sequences.
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