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In addition, we assessed the activation of mitogen-activated protein kinase (MAPK) pathways.
Finally, we assessed the activation of NF-κB and activating protein-1, as well as phosphorylation of p38 mitogen-activated protein kinase by electrophoretic mobility shift assay with super shift and/or Western blot.
We initially assessed the activation of the GTPase by capturing the activated form of RalA on a GST-affinity column containing a fragment of Ral binding protein 1 (RalBP1) [19].
We assessed the activation of the STAT3 (signal transducer and activator of transcription 3) signalling pathway and the downstream targets in the cells with different expression levels of NS.
Finally, we assessed the activation of the terminal end of the signaling pathway by measuring the relative activity of a luciferase reporter under the control of the NF-κB promoter in HEK 293 cells transfected with expression vectors containing hMD-2, human TLR4 (hTLR4) and human CD14 (hCD14).
We subjected OLs to glucose/nutrient deprivation for 35 h to simulate ischemic metabolic stress associated with an MS plaque and assessed the activation of caspases 3/7.
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The modulation of the ERN amplitude in relation to memory decisions was interpreted as reflecting the activity of an internal monitoring device assessing the activation of the two possible decisions [29].
The aim of this study was to assess the activation of superficial brain structures by popular dance music and art music in young adults using functional magnetic resonance imaging.
The present study was designed to assess the activation of key neuronal populations in subdivisions of the accumbens and subdivisions of the medial prefrontal cortex in cocaine-sensitized rats, using the expression of the immediate early gene, c-fos, as a marker of neuronal activation.
Were any intermediate outcome measures taken to assess the activation of the causal mechanism(s) judged to underpin the intervention?
Anti-ED1 immunostaining was performed to assess the activation of microglia.
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