Exact(11)
We next assessed T cell activation levels by monitoring CD25 expression and proliferation in primary T cells expressing wild-type and uncleavable CTFs.
To test whether a similar effect is seen in vivo, we immunized mice with CD8α+ DC and monocytes that were co-infected ex vivo and assessed T cell responses via CFSE dilution.
After finding significant neonatal T cell activation following preterm delivery, we assessed T cell activation in infants delivered to mothers with a diagnosis of clinical chorioamnionitis, defined as the presence of maternal fever, tachycardia, and uterine tenderness and a decision by the treating physician to institute intrapartum parenteral antibiotics.
We initially assessed T cell responses in patients and control subjects at the index visit, when patients had active uveitis.
43 We assessed T cell cross-reactivity in patients with resolved infection first using genotype-specific overlapping peptides and showed that cross-reactivity was minimal.
First, we assessed T cell cross-reactivity in patients with resolved infection using genotype-specific overlapping peptides across the whole genome.
Similar(48)
As secondary outcomes, we also assessed T-cell and antibody responses.
Initially, we stimulated primary human T cells with CD3×CD28-coated microbeads for 16 hours in the presence of antioxidants and assessed T-cell activation.
We assessed T-lymphocyte proliferation using cryopreserved PBMCs with two methods: H-thymidine incorporation in response to the mitogen phytohemagglutinin (PHA), and the cell-division tracking method in response to anti-CD3 antibody.
Most routinely assessed T-cell diagnostic markers (e.g. TCR-α/β, CD3, CD4, CD8 and CD45) are components of the antigen receptor complex and are dynamically regulated during T-cell maturation and activation [ 13].
A single-arm, multi-center trial assessed T-DM1 as a single agent in 110 women with HER2-positive advanced breast cancer whose disease had progressed after prior HER2-targeted therapy and chemotherapy.
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