Sentence examples for assessed sphere from inspiring English sources

Considering that cell aggregation might confuse true sphere formation in liquid culture, we further assessed sphere formation in methylcellulose-containing semisolid culture to rule out this confusion.

We then seeded one cell from each subpopulation in individual wells of a 96-well plate and assessed sphere formation after 14 days.

Adult SVZ NS were sorted into a 96-well plate containing medium at 20 cells per well and allowed to grow for 2 weeks before assessing sphere formation.

Thus, this assay can assess sphere proliferation.

To assess sphere forming capacity, cells were trypsinized and plated in sphere media (DMEM-F12, 0.4% BSA (Sigma), 10 ml/500 ml B27 (Invitrogen) 5 μg/ml bovine insulin (Sigma), 4 μg/ml heparin (Sigma), 20 ng/ml fibroblast growth factor 2 (bFGF, Sigma) and 20 ng/ml epidermal growth factor (EGF, Sigma)) into 96-well ultra-low adhesion plates, ranging from 1 to 256 cells/well.

To assess sphere-forming efficiency after estrogen treatment or endocrinolectomy, pituitaries were harvested 3 and 6 days after surgery, respectively, and spheres were counted blindly and manually after 1 week in culture.

To assess sphere-forming ability in serum and non-serum containing media in nonadherent conditions, single FACS-sorted CD133+ or CD133- cells were seeded into low attachment 96-well plates.

In this study, we have demonstrated that CDF not only inhibit cell growth of PC cells, but also inhibit CSC self-renewal capacity as assessed by sphere formation (pancreatospheres) assays.

One of the most important characteristics of CSCs is self-renewal ability, which is assessed by sphere formation.

We assessed the sphere-forming ability.

But what's most important for all of this, in my mind, is to accurately assess your sphere of influence and work within that sphere of influence.